The Italian sat in the dugout for the first time on the weekend as the Hornets drew 1-1 with Ipswich Town at Portman Road.
A win for Watford could see the team move into the top half of the table ahead of a busy festive period.
Meanwhile, Millwall will be keen to pick up a positive result as they sit just four points above the relegation zone following two straight defeats.Wing-back Pudil was forced off as a precaution nursing a hamstring complaint in the latter stages of Saturday's 1-1 draw at Ipswich - new head coach Giuseppe Sannino's first match at the helm since taking over from Gianfranco Zola.
Versatile on-loan Arsenal right-back Bellerin missed the encounter altogether with a minor hip injury and will be assessed.
Marco Faraoni (ankle), Almen Abdi (foot), Essaid Belkalem and Tommie Hoban (both ankle) remain sidelined as Sannino takes charge of his first home game.
Millwall, meanwhile, will check on skipper Paul Robinson ahead of the trip to Watford.
The defender needed stitches in an ankle wound following Saturday's 2-0 home defeat by Middlesbrough.